1 step Search Results


qscript xlt 1 step rt qpcr toughmix  (Quanta Biosciences)
96
Quanta Biosciences qscript xlt 1 step rt qpcr toughmix
Qscript Xlt 1 Step Rt Qpcr Toughmix, supplied by Quanta Biosciences, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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time rt pcr kit qpcrbio probe 1 step  (PCR Biosystems Ltd)
95
PCR Biosystems Ltd time rt pcr kit qpcrbio probe 1 step
Time Rt Pcr Kit Qpcrbio Probe 1 Step, supplied by PCR Biosystems Ltd, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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qpcrbio sygreen 1 step detect hi rox mix  (PCR Biosystems Ltd)
95
PCR Biosystems Ltd qpcrbio sygreen 1 step detect hi rox mix
Qpcrbio Sygreen 1 Step Detect Hi Rox Mix, supplied by PCR Biosystems Ltd, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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qscript lyo 1 step  (Quanta Biosciences)
96
Quanta Biosciences qscript lyo 1 step
Qscript Lyo 1 Step, supplied by Quanta Biosciences, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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one step luciferase assay system  (BPS Bioscience)
97
BPS Bioscience one step luciferase assay system
One Step Luciferase Assay System, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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homogenization step  (Proteintech)
96
Proteintech homogenization step
HiTmiSS assay and analysis on mixed tissue. ( A ) HiTmiSS protocol for RNA extraction, extension, reverse transcription, and quantification in high throughput. Steps include (i) C. elegans growth and <t>homogenization,</t> (ii) RNA extraction and isolation, (iii) RNA polyuridinylation, extension, and reverse transcription (details shown in ii; orange: miRNAs, green: polyU, purple: SL-polyA reverse universal primer), (iv) addition of 5p or 3p miRNA-strand-specific forward primers, (v) loading into 384-well plates, and (vi) quantification. ( B ) Heatmap of miRNA expression ( n = 3) in mixed tissue clustered by quantity, strand use, and developmental stage. Stages include embryo ( E ), larval 1 through 4 (L1-L4), and YA. The heatmap legend represents the log 10 of expression values for each miRNA strand, normalized by the spike-in GFP control, at each stage, ranging from −4 (deep purple) to 4 (bright yellow). Black color indicates instances of data that fall below the strict expression threshold (one standard deviation above the mean of all expression values at that stage). ( C ) Heatmap of pairing frequency by position for the used (left) and discarded (right) strand of the 20 most highly quantified 5p and 3p Singletons in our mixed tissue dataset. (i) blue: paired; red: unpaired). (ii) Average pairing frequency, and logo plot of nucleotide frequency at each position displayed below (gold box marks the seed regions).
Homogenization Step, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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homogenization step - by Bioz Stars, 2026-03
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1 step  (PCR Biosystems Ltd)
94
PCR Biosystems Ltd 1 step
HiTmiSS assay and analysis on mixed tissue. ( A ) HiTmiSS protocol for RNA extraction, extension, reverse transcription, and quantification in high throughput. Steps include (i) C. elegans growth and <t>homogenization,</t> (ii) RNA extraction and isolation, (iii) RNA polyuridinylation, extension, and reverse transcription (details shown in ii; orange: miRNAs, green: polyU, purple: SL-polyA reverse universal primer), (iv) addition of 5p or 3p miRNA-strand-specific forward primers, (v) loading into 384-well plates, and (vi) quantification. ( B ) Heatmap of miRNA expression ( n = 3) in mixed tissue clustered by quantity, strand use, and developmental stage. Stages include embryo ( E ), larval 1 through 4 (L1-L4), and YA. The heatmap legend represents the log 10 of expression values for each miRNA strand, normalized by the spike-in GFP control, at each stage, ranging from −4 (deep purple) to 4 (bright yellow). Black color indicates instances of data that fall below the strict expression threshold (one standard deviation above the mean of all expression values at that stage). ( C ) Heatmap of pairing frequency by position for the used (left) and discarded (right) strand of the 20 most highly quantified 5p and 3p Singletons in our mixed tissue dataset. (i) blue: paired; red: unpaired). (ii) Average pairing frequency, and logo plot of nucleotide frequency at each position displayed below (gold box marks the seed regions).
1 Step, supplied by PCR Biosystems Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/1 step/product/PCR Biosystems Ltd
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transcriptase pcr kit qscripttm xlt 1 step rt pcr  (Quanta Biosciences)
95
Quanta Biosciences transcriptase pcr kit qscripttm xlt 1 step rt pcr
HiTmiSS assay and analysis on mixed tissue. ( A ) HiTmiSS protocol for RNA extraction, extension, reverse transcription, and quantification in high throughput. Steps include (i) C. elegans growth and <t>homogenization,</t> (ii) RNA extraction and isolation, (iii) RNA polyuridinylation, extension, and reverse transcription (details shown in ii; orange: miRNAs, green: polyU, purple: SL-polyA reverse universal primer), (iv) addition of 5p or 3p miRNA-strand-specific forward primers, (v) loading into 384-well plates, and (vi) quantification. ( B ) Heatmap of miRNA expression ( n = 3) in mixed tissue clustered by quantity, strand use, and developmental stage. Stages include embryo ( E ), larval 1 through 4 (L1-L4), and YA. The heatmap legend represents the log 10 of expression values for each miRNA strand, normalized by the spike-in GFP control, at each stage, ranging from −4 (deep purple) to 4 (bright yellow). Black color indicates instances of data that fall below the strict expression threshold (one standard deviation above the mean of all expression values at that stage). ( C ) Heatmap of pairing frequency by position for the used (left) and discarded (right) strand of the 20 most highly quantified 5p and 3p Singletons in our mixed tissue dataset. (i) blue: paired; red: unpaired). (ii) Average pairing frequency, and logo plot of nucleotide frequency at each position displayed below (gold box marks the seed regions).
Transcriptase Pcr Kit Qscripttm Xlt 1 Step Rt Pcr, supplied by Quanta Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ct 1 step kit  (Thermo Fisher)
96
Thermo Fisher ct 1 step kit
HiTmiSS assay and analysis on mixed tissue. ( A ) HiTmiSS protocol for RNA extraction, extension, reverse transcription, and quantification in high throughput. Steps include (i) C. elegans growth and <t>homogenization,</t> (ii) RNA extraction and isolation, (iii) RNA polyuridinylation, extension, and reverse transcription (details shown in ii; orange: miRNAs, green: polyU, purple: SL-polyA reverse universal primer), (iv) addition of 5p or 3p miRNA-strand-specific forward primers, (v) loading into 384-well plates, and (vi) quantification. ( B ) Heatmap of miRNA expression ( n = 3) in mixed tissue clustered by quantity, strand use, and developmental stage. Stages include embryo ( E ), larval 1 through 4 (L1-L4), and YA. The heatmap legend represents the log 10 of expression values for each miRNA strand, normalized by the spike-in GFP control, at each stage, ranging from −4 (deep purple) to 4 (bright yellow). Black color indicates instances of data that fall below the strict expression threshold (one standard deviation above the mean of all expression values at that stage). ( C ) Heatmap of pairing frequency by position for the used (left) and discarded (right) strand of the 20 most highly quantified 5p and 3p Singletons in our mixed tissue dataset. (i) blue: paired; red: unpaired). (ii) Average pairing frequency, and logo plot of nucleotide frequency at each position displayed below (gold box marks the seed regions).
Ct 1 Step Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ct 1 step kit - by Bioz Stars, 2026-03
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cybrfast 1 step rt qpcr lo rox kit  (Cytek Biosciences)
93
Cytek Biosciences cybrfast 1 step rt qpcr lo rox kit
HiTmiSS assay and analysis on mixed tissue. ( A ) HiTmiSS protocol for RNA extraction, extension, reverse transcription, and quantification in high throughput. Steps include (i) C. elegans growth and <t>homogenization,</t> (ii) RNA extraction and isolation, (iii) RNA polyuridinylation, extension, and reverse transcription (details shown in ii; orange: miRNAs, green: polyU, purple: SL-polyA reverse universal primer), (iv) addition of 5p or 3p miRNA-strand-specific forward primers, (v) loading into 384-well plates, and (vi) quantification. ( B ) Heatmap of miRNA expression ( n = 3) in mixed tissue clustered by quantity, strand use, and developmental stage. Stages include embryo ( E ), larval 1 through 4 (L1-L4), and YA. The heatmap legend represents the log 10 of expression values for each miRNA strand, normalized by the spike-in GFP control, at each stage, ranging from −4 (deep purple) to 4 (bright yellow). Black color indicates instances of data that fall below the strict expression threshold (one standard deviation above the mean of all expression values at that stage). ( C ) Heatmap of pairing frequency by position for the used (left) and discarded (right) strand of the 20 most highly quantified 5p and 3p Singletons in our mixed tissue dataset. (i) blue: paired; red: unpaired). (ii) Average pairing frequency, and logo plot of nucleotide frequency at each position displayed below (gold box marks the seed regions).
Cybrfast 1 Step Rt Qpcr Lo Rox Kit, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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astrovirus  (Quanta Biosciences)
94
Quanta Biosciences astrovirus
HiTmiSS assay and analysis on mixed tissue. ( A ) HiTmiSS protocol for RNA extraction, extension, reverse transcription, and quantification in high throughput. Steps include (i) C. elegans growth and <t>homogenization,</t> (ii) RNA extraction and isolation, (iii) RNA polyuridinylation, extension, and reverse transcription (details shown in ii; orange: miRNAs, green: polyU, purple: SL-polyA reverse universal primer), (iv) addition of 5p or 3p miRNA-strand-specific forward primers, (v) loading into 384-well plates, and (vi) quantification. ( B ) Heatmap of miRNA expression ( n = 3) in mixed tissue clustered by quantity, strand use, and developmental stage. Stages include embryo ( E ), larval 1 through 4 (L1-L4), and YA. The heatmap legend represents the log 10 of expression values for each miRNA strand, normalized by the spike-in GFP control, at each stage, ranging from −4 (deep purple) to 4 (bright yellow). Black color indicates instances of data that fall below the strict expression threshold (one standard deviation above the mean of all expression values at that stage). ( C ) Heatmap of pairing frequency by position for the used (left) and discarded (right) strand of the 20 most highly quantified 5p and 3p Singletons in our mixed tissue dataset. (i) blue: paired; red: unpaired). (ii) Average pairing frequency, and logo plot of nucleotide frequency at each position displayed below (gold box marks the seed regions).
Astrovirus, supplied by Quanta Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/astrovirus/product/Quanta Biosciences
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illustra exo prostar 1 step  (GE Healthcare)
93
GE Healthcare illustra exo prostar 1 step
HiTmiSS assay and analysis on mixed tissue. ( A ) HiTmiSS protocol for RNA extraction, extension, reverse transcription, and quantification in high throughput. Steps include (i) C. elegans growth and <t>homogenization,</t> (ii) RNA extraction and isolation, (iii) RNA polyuridinylation, extension, and reverse transcription (details shown in ii; orange: miRNAs, green: polyU, purple: SL-polyA reverse universal primer), (iv) addition of 5p or 3p miRNA-strand-specific forward primers, (v) loading into 384-well plates, and (vi) quantification. ( B ) Heatmap of miRNA expression ( n = 3) in mixed tissue clustered by quantity, strand use, and developmental stage. Stages include embryo ( E ), larval 1 through 4 (L1-L4), and YA. The heatmap legend represents the log 10 of expression values for each miRNA strand, normalized by the spike-in GFP control, at each stage, ranging from −4 (deep purple) to 4 (bright yellow). Black color indicates instances of data that fall below the strict expression threshold (one standard deviation above the mean of all expression values at that stage). ( C ) Heatmap of pairing frequency by position for the used (left) and discarded (right) strand of the 20 most highly quantified 5p and 3p Singletons in our mixed tissue dataset. (i) blue: paired; red: unpaired). (ii) Average pairing frequency, and logo plot of nucleotide frequency at each position displayed below (gold box marks the seed regions).
Illustra Exo Prostar 1 Step, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/illustra exo prostar 1 step/product/GE Healthcare
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Image Search Results


HiTmiSS assay and analysis on mixed tissue. ( A ) HiTmiSS protocol for RNA extraction, extension, reverse transcription, and quantification in high throughput. Steps include (i) C. elegans growth and homogenization, (ii) RNA extraction and isolation, (iii) RNA polyuridinylation, extension, and reverse transcription (details shown in ii; orange: miRNAs, green: polyU, purple: SL-polyA reverse universal primer), (iv) addition of 5p or 3p miRNA-strand-specific forward primers, (v) loading into 384-well plates, and (vi) quantification. ( B ) Heatmap of miRNA expression ( n = 3) in mixed tissue clustered by quantity, strand use, and developmental stage. Stages include embryo ( E ), larval 1 through 4 (L1-L4), and YA. The heatmap legend represents the log 10 of expression values for each miRNA strand, normalized by the spike-in GFP control, at each stage, ranging from −4 (deep purple) to 4 (bright yellow). Black color indicates instances of data that fall below the strict expression threshold (one standard deviation above the mean of all expression values at that stage). ( C ) Heatmap of pairing frequency by position for the used (left) and discarded (right) strand of the 20 most highly quantified 5p and 3p Singletons in our mixed tissue dataset. (i) blue: paired; red: unpaired). (ii) Average pairing frequency, and logo plot of nucleotide frequency at each position displayed below (gold box marks the seed regions).

Journal: Nucleic Acids Research

Article Title: An AI-guided framework reveals conserved features governing microRNA strand selection

doi: 10.1093/nar/gkaf1510

Figure Lengend Snippet: HiTmiSS assay and analysis on mixed tissue. ( A ) HiTmiSS protocol for RNA extraction, extension, reverse transcription, and quantification in high throughput. Steps include (i) C. elegans growth and homogenization, (ii) RNA extraction and isolation, (iii) RNA polyuridinylation, extension, and reverse transcription (details shown in ii; orange: miRNAs, green: polyU, purple: SL-polyA reverse universal primer), (iv) addition of 5p or 3p miRNA-strand-specific forward primers, (v) loading into 384-well plates, and (vi) quantification. ( B ) Heatmap of miRNA expression ( n = 3) in mixed tissue clustered by quantity, strand use, and developmental stage. Stages include embryo ( E ), larval 1 through 4 (L1-L4), and YA. The heatmap legend represents the log 10 of expression values for each miRNA strand, normalized by the spike-in GFP control, at each stage, ranging from −4 (deep purple) to 4 (bright yellow). Black color indicates instances of data that fall below the strict expression threshold (one standard deviation above the mean of all expression values at that stage). ( C ) Heatmap of pairing frequency by position for the used (left) and discarded (right) strand of the 20 most highly quantified 5p and 3p Singletons in our mixed tissue dataset. (i) blue: paired; red: unpaired). (ii) Average pairing frequency, and logo plot of nucleotide frequency at each position displayed below (gold box marks the seed regions).

Article Snippet: During this homogenization step, anti-GFP magnetic antibody beads (Chromotek GFP-Trap Magnetic Agarose) were prepared by diluting 25 μl bead slurry in 500 μl dilution buffer (150 mM NaCl, 50 mM Tris–HCl, pH 8.0, 0.5 mM EDTA) and rotating end-over-end at 4°C for 3 min.

Techniques: RNA Extraction, Reverse Transcription, High Throughput Screening Assay, Homogenization, Isolation, Expressing, Control, Standard Deviation